Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

New interaction partners for Nek4.1 and Nek4.2 isoforms: from the DNA damage response to RNA splicing.

Basei FL, Meirelles GV, Righetto GL, Dos Santos Migueleti DL, Smetana JH, Kobarg J

Neks are serine-threonine kinases that are similar to NIMA, a protein found in Aspergillus nidulans which is essential for cell division. In humans there are eleven Neks which are involved in different biological functions besides the cell cycle control. Nek4 is one of the largest members of the Nek family and has been related to the primary cilia formation and ... [more]

Unknown Mar. 24, 2015; 13();11 [Pubmed: 25798074]

Throughput

  • High Throughput

Curated By

  • BioGRID