FOXH1
Gene Ontology Biological Process
- negative regulation of androgen receptor activity [IDA]
- negative regulation of androgen receptor signaling pathway [IDA]
- negative regulation of intracellular estrogen receptor signaling pathway [IDA]
- nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry [NAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- transforming growth factor beta receptor signaling pathway [ISS, TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- R-SMAD binding [IMP, IPI]
- SMAD binding [IPI]
- androgen receptor binding [IPI]
- bHLH transcription factor binding [IPI]
- co-SMAD binding [IMP]
- enhancer binding [IC]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription regulatory region DNA binding [ISS]
- DNA binding [IDA]
- R-SMAD binding [IMP, IPI]
- SMAD binding [IPI]
- androgen receptor binding [IPI]
- bHLH transcription factor binding [IPI]
- co-SMAD binding [IMP]
- enhancer binding [IC]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
DRAP1
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Inhibition of excess nodal signaling during mouse gastrulation by the transcriptional corepressor DRAP1.
The formation and patterning of mesoderm during mammalian gastrulation require the activity of Nodal, a secreted mesoderm-inducing factor of the transforming growth factor-beta (TGF-beta) family. Here we show that the transcriptional corepressor DRAP1 has a very specific role in regulation of Nodal activity during mouse embryogenesis. We find that loss of Drap1 leads to severe gastrulation defects that are consistent ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FOXH1 DRAP1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID