Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cooperation of six and eya in activation of their target genes through nuclear translocation of Eya.

Ohto H, Kamada S, Tago K, Tominaga SI, Ozaki H, Sato S, Kawakami K

Drosophila sine oculis and eyes absent genes synergize in compound-eye formation. The murine homologues of these genes, Six and Eya, respectively, show overlapping expression patterns during development. We hypothesized that Six and Eya proteins cooperate to regulate their target genes. Cotransfection assays were performed with various combinations of Six and Eya to assess their effects on a potential natural target, ... [more]

Mol. Cell. Biol. Oct. 01, 1999; 19(10);6815-24 [Pubmed: 10490620]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EYA2 SIX4	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	St-Denis N (2016)	High	-	BioGRID	2214890

Curated By

BioGRID

Interaction Summary

EYA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

magnesium ion binding [IDA]protein binding [IPI]protein tyrosine phosphatase activity [IDA]

Gene Ontology Cellular Component

SIX4