PREY

TRIM63

IRF, MURF1, MURF2, RNF28, SMRZ
tripartite motif containing 63, E3 ubiquitin protein ligase
GO Process (2)
GO Function (3)
GO Component (2)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1.

McElhinny AS, Kakinuma K, Sorimachi H, Labeit S, Gregorio CC

The COOH-terminal A168-170 region of the giant sarcomeric protein titin interacts with muscle-specific RING finger-1 (MURF-1). To investigate the functional significance of this interaction, we expressed green fluorescent protein fusion constructs encoding defined fragments of titin's M-line region and MURF-1 in cardiac myocytes. Upon expression of MURF-1 or its central region (containing its titin-binding site), the integrity of titin's M-line ... [more]

J. Cell Biol. Apr. 01, 2002; 157(1);125-36 [Pubmed: 11927605]

Throughput

  • Low Throughput

Curated By

  • BioGRID