An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle Differentiation.

Ansseau E, Eidahl JO, Lancelot C, Tassin A, Matteotti C, Yip C, Liu J, Leroy B, Hubeau C, Gerbaux C, Cloet S, Wauters A, Zorbo S, Meyer P, Pirson I, Laoudj-Chenivesse D, Wattiez R, Harper SQ, Belayew A, Coppee F

Hundreds of double homeobox (DUX) genes map within 3.3-kb repeated elements dispersed in the human genome and encode DNA-binding proteins. Among these, we identified DUX4, a potent transcription factor that causes facioscapulohumeral muscular dystrophy (FSHD). In the present study, we performed yeast two-hybrid screens and protein co-purifications with HaloTag-DUX fusions or GST-DUX4 pull-down to identify protein partners of DUX4, DUX4c ... [more]

Unknown Jan. 28, 2016; 11(1);e0146893 [Pubmed: 26816005]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DUX4 RPL7	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ansseau E (2016)	High	-	BioGRID	2822861

Curated By

BioGRID

Interaction Summary

DUX4

Gene Ontology Biological Process

Gene Ontology Molecular Function

transcription regulatory region sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

RPL7