BAIT

ATF4

CREB-2, CREB2, TAXREB67, TXREB
activating transcription factor 4
GO Process (20)
GO Function (7)
GO Component (6)
Homo sapiens
PREY

EIF2A

EIF-2A, MST089, MSTP004, MSTP089, CDA02
eukaryotic translation initiation factor 2A, 65kDa
GO Process (2)
GO Function (4)
GO Component (4)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Glucose-stimulated protein synthesis in pancreatic beta-cells parallels an increase in the availability of the translational ternary complex (eIF2-GTP.Met-tRNAi) and the dephosphorylation of eIF2 alpha.

Gomez E, Powell ML, Greenman IC, Herbert TP

In pancreatic beta-cells, glucose causes a rapid increase in the rate of protein synthesis. However, the mechanism by which this occurs is poorly understood. In this report, we demonstrate, in the pancreatic beta-cell line MIN6, that glucose stimulates the recruitment of ribosomes onto the mRNA, indicative of an increase in the rate of the initiation step of protein synthesis. This ... [more]

J. Biol. Chem. Dec. 24, 2004; 279(52);53937-46 [Pubmed: 15475356]

Throughput

  • Low Throughput

Curated By

  • BioGRID