BAIT
HTR2C
5-HT2C, 5-HTR2C, 5HTR2C, HTR1C, RP11-810O3.1
5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
GO Process (16)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- behavioral fear response [ISS]
- cGMP biosynthetic process [IDA]
- cellular calcium ion homeostasis [IDA]
- feeding behavior [ISS]
- phospholipase C-activating G-protein coupled receptor signaling pathway [IDA]
- phospholipase C-activating serotonin receptor signaling pathway [ISS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [ISS]
- positive regulation of phosphatidylinositol biosynthetic process [IDA]
- regulation of appetite [ISS]
- regulation of corticotropin-releasing hormone secretion [ISS]
- regulation of neurological system process [ISS]
- release of sequestered calcium ion into cytosol [IMP]
- response to drug [IDA]
- serotonin receptor signaling pathway [IMP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GPRC5B
RAIG-2, RAIG2
G protein-coupled receptor, class C, group 5, member B
GO Process (6)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISS]
- positive regulation of canonical Wnt signaling pathway [ISS]
- positive regulation of inflammatory response [ISS]
- positive regulation of macrophage cytokine production [ISS]
- positive regulation of neuron differentiation [ISS]
- positive regulation of protein tyrosine kinase activity [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]
Mol. Syst. Biol. Dec. 15, 2016; 13(3);918 [Pubmed: 28298427]
Throughput
- High Throughput
Additional Notes
- interaction identified using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) assay
Curated By
- BioGRID