BAIT

PDGFA

PDGF-A, PDGF1
platelet-derived growth factor alpha polypeptide
GO Process (44)
GO Function (7)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens
PREY

PLCG1

NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, RP3-511B24.2
phospholipase C, gamma 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phosphorylation sites at the C-terminus of the platelet-derived growth factor receptor bind phospholipase C gamma 1.

Kashishian A, Cooper JA

We have identified two tyrosine phosphorylation sites, Tyr 1009 and Tyr 1021, in the C-terminal noncatalytic region of the human platelet-derived growth factor (PDGF) receptor beta subunit. Mutant receptors with phenylalanine substitutions at either or both of these tyrosines were expressed in dog epithelial cells. Mutation of Tyr 1021 markedly reduced the PDGF-stimulated binding of phospholipase C (PLC) gamma 1 ... [more]

Mol. Biol. Cell Jan. 01, 1993; 4(1);49-57 [Pubmed: 8443409]

Throughput

  • Low Throughput

Curated By

  • BioGRID