BAIT

CCR1

CD191, CKR-1, CKR1, CMKBR1, HM145, MIP1aR, SCYAR1
chemokine (C-C motif) receptor 1
Homo sapiens
PREY

FAN1

KIAA1018, KMIN, MTMR15, RP11-540B6.6
FANCD2/FANCI-associated nuclease 1
Fanconi Anemia Project
UPS Project
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.

Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gandia J, Benleulmi-Chaachoua A, Tadagaki K, Oishi A, Wong V, Malty RH, Deineko V, Aoki H, Amin S, Yao Z, Morato X, Otasek D, Kobayashi H, Menendez J, Auerbach D, Angers S, Przulj N, Bouvier M, Babu M, Ciruela F, Jockers R, Jurisica I, Stagljar I

G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]

Mol. Syst. Biol. Dec. 15, 2016; 13(3);918 [Pubmed: 28298427]

Throughput

  • High Throughput

Additional Notes

  • interaction identified using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) assay

Curated By

  • BioGRID