Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

A novel Rab9 effector required for endosome-to-TGN transport.

Diaz E, Schimmoeller F, Pfeffer SR

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. We have used the yeast two-hybrid system to identify proteins that interact preferentially with the active form of Rab9. We report here the discovery of a 40-kD protein (p40) that ... [more]

J. Cell Biol. Jul. 28, 1997; 138(2);283-90 [Pubmed: 9230071]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAB9A RABEPK
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID