BAIT
CHRM5
HM5
cholinergic receptor, muscarinic 5
GO Process (4)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
USP33
VDU1
ubiquitin specific peptidase 33
GO Process (10)
GO Function (7)
GO Component (5)
Gene Ontology Biological Process
- axon guidance [ISS]
- cell migration [ISS]
- centrosome duplication [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [IDA]
- protein K63-linked deubiquitination [IDA]
- protein deubiquitination [IDA]
- regulation of G-protein coupled receptor protein signaling pathway [IMP]
- regulation of proteasomal protein catabolic process [IBA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]
Mol. Syst. Biol. Dec. 15, 2016; 13(3);918 [Pubmed: 28298427]
Throughput
- High Throughput
Additional Notes
- interaction identified using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) assay
Curated By
- BioGRID