BAIT

PLCG1

NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, RP3-511B24.2
phospholipase C, gamma 1
Homo sapiens
PREY

PAK1

PAKalpha
p21 protein (Cdc42/Rac)-activated kinase 1
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Basic fibroblast growth factor-induced translocation of p21-activated kinase to the membrane is independent of phospholipase C-gamma1 in the differentiation of PC12 cells.

Shin KS, Shin EY, Lee CS, Quan SH, Woo KN, Soung NK, Kwak SJ, Kim SR, Kim EG

p21-activated kinase (PAK) targeting to the plasma membrane is essential for PC12 cell neurite outgrowth. Phospholipase C-gamma1 (PLC-gamma1) can mediate the PAK translocation in response to growth factors, since PLC-gamma1 binds to both tyrosine-phosphorylated receptor tyrosine kinases and PAK through its SH2 and SH3 domain, respectively. In the present study, we examined a potential role for PLC-gamma1 in the basic ... [more]

Exp. Mol. Med. May. 31, 2002; 34(2);172-6 [Pubmed: 12085993]

Throughput

  • Low Throughput

Curated By

  • BioGRID