BAIT
CHRM5
HM5
cholinergic receptor, muscarinic 5
GO Process (4)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EPC1
Epl1
enhancer of polycomb homolog 1 (Drosophila)
GO Process (9)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
- chromatin organization [TAS]
- histone H2A acetylation [IDA]
- histone H4 acetylation [IDA]
- negative regulation of gene expression, epigenetic [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [TAS]
- transcription, DNA-templated [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]
Mol. Syst. Biol. Dec. 15, 2016; 13(3);918 [Pubmed: 28298427]
Throughput
- High Throughput
Additional Notes
- interaction identified using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) assay
Curated By
- BioGRID