BAIT

AP3S1

CLAPS3, Sigma3A
adaptor-related protein complex 3, sigma 1 subunit
GO Process (3)
GO Function (2)
GO Component (3)
Homo sapiens
PREY

IRS1

HIRS-1
insulin receptor substrate 1
GO Process (25)
GO Function (7)
GO Component (7)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interaction of insulin receptor substrate-1 with the sigma3A subunit of the adaptor protein complex-3 in cultured adipocytes.

VanRenterghem B, Morin M, Czech MP, Heller-Harrison RA

Signaling through the insulin receptor tyrosine kinase involves its autophosphorylation in response to insulin and the subsequent tyrosine phosphorylation of substrate proteins such as insulin receptor substrate-1 (IRS-1). In basal 3T3-L1 adipocytes, IRS-1 is predominantly membrane-bound, and this localization may be important in targeting downstream signaling elements that mediate insulin action. Since IRS-1 localization to membranes may occur through its ... [more]

J. Biol. Chem. Nov. 06, 1998; 273(45);29942-9 [Pubmed: 9792713]

Throughput

  • Low Throughput

Curated By

  • BioGRID