Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional genomic landscape of cancer-intrinsic evasion of killing by T cells.

Lawson KA, Sousa CM, Zhang X, Kim E, Akthar R, Caumanns JJ, Yao Y, Mikolajewicz N, Ross C, Brown KR, Zid AA, Fan ZP, Hui S, Krall JA, Simons DM, Slater CJ, De Jesus V, Tang L, Singh R, Goldford JE, Martin S, Huang Q, Francis EA, Habsid A, Climie R, Tieu D, Wei J, Li R, Tong AHY, Aregger M, Chan KS, Han H, Wang X, Mero P, Brumell JH, Finelli A, Ailles L, Bader G, Smolen GA, Kingsbury GA, Hart T, Kung C, Moffat J

The genetic circuits that allow cancer cells to evade destruction by the host immune system remain poorly understood1-3. Here, to identify a phenotypically robust core set of genes and pathways that enable cancer cells to evade killing mediated by cytotoxic T lymphocytes (CTLs), we performed genome-wide CRISPR screens across a panel of genetically diverse mouse cancer cell lines that were cultured ... [more]

Nature Oct. 01, 2020; 586(7827);120-126 [Pubmed: 32968282]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: decreased cell proliferation (MP:0000352)

Additional Notes

  • CRISPR GI screen
  • Cell Line: Renca
  • Experimental Setup: Cytotoxic T cell exposure
  • GIST: A-phenotypic negative genetic interaction
  • Library: mTKO
  • Significance Threshold: FDR<0.05

Curated By

  • BioGRID