BAIT

ATG12

4931423H11Rik, A330058M13Rik, Apg12l, Atg12l
autophagy related 12
Mus musculus
PREY

ATG16L1

1500009K01Rik, Apg16l, Atg16l, WDR30
autophagy related 16-like 1 (S. cerevisiae)
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional genomic landscape of cancer-intrinsic evasion of killing by T cells.

Lawson KA, Sousa CM, Zhang X, Kim E, Akthar R, Caumanns JJ, Yao Y, Mikolajewicz N, Ross C, Brown KR, Zid AA, Fan ZP, Hui S, Krall JA, Simons DM, Slater CJ, De Jesus V, Tang L, Singh R, Goldford JE, Martin S, Huang Q, Francis EA, Habsid A, Climie R, Tieu D, Wei J, Li R, Tong AHY, Aregger M, Chan KS, Han H, Wang X, Mero P, Brumell JH, Finelli A, Ailles L, Bader G, Smolen GA, Kingsbury GA, Hart T, Kung C, Moffat J

The genetic circuits that allow cancer cells to evade destruction by the host immune system remain poorly understood1-3. Here, to identify a phenotypically robust core set of genes and pathways that enable cancer cells to evade killing mediated by cytotoxic T lymphocytes (CTLs), we performed genome-wide CRISPR screens across a panel of genetically diverse mouse cancer cell lines that were cultured ... [more]

Nature Oct. 01, 2020; 586(7827);120-126 [Pubmed: 32968282]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: increased cell proliferation (MP:0000351)

Additional Notes

  • CRISPR GI screen
  • Cell Line: Renca
  • Experimental Setup: Cytotoxic T cell exposure
  • Experimental Setup: TNF exposure
  • GIST: A-phenotypic positive genetic interaction
  • Library: mTKO
  • Significance Threshold: FDR<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATG16L1 ATG12
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
ATG16L1 ATG12
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.967BioGRID
2666985

Curated By

  • BioGRID