Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional genomic landscape of cancer-intrinsic evasion of killing by T cells.

Lawson KA, Sousa CM, Zhang X, Kim E, Akthar R, Caumanns JJ, Yao Y, Mikolajewicz N, Ross C, Brown KR, Zid AA, Fan ZP, Hui S, Krall JA, Simons DM, Slater CJ, De Jesus V, Tang L, Singh R, Goldford JE, Martin S, Huang Q, Francis EA, Habsid A, Climie R, Tieu D, Wei J, Li R, Tong AHY, Aregger M, Chan KS, Han H, Wang X, Mero P, Brumell JH, Finelli A, Ailles L, Bader G, Smolen GA, Kingsbury GA, Hart T, Kung C, Moffat J

The genetic circuits that allow cancer cells to evade destruction by the host immune system remain poorly understood1-3. Here, to identify a phenotypically robust core set of genes and pathways that enable cancer cells to evade killing mediated by cytotoxic T lymphocytes (CTLs), we performed genome-wide CRISPR screens across a panel of genetically diverse mouse cancer cell lines that were cultured ... [more]

Nature Oct. 01, 2020; 586(7827);120-126 [Pubmed: 32968282]

Throughput

Low Throughput

Ontology Terms

increased cell proliferation (MP:0000351)

Additional Notes

CRISPR GI screen
Cell Line: Renca
Experimental Setup: TNF exposure
GIST: A-phenotypic positive genetic interaction
Library: mTKO
Significance Threshold: FDR<0.05

Curated By

BioGRID

Interaction Summary

ATG12

Gene Ontology Biological Process

Gene Ontology Molecular Function

Atg8 ligase activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component

USP9X

Gene Ontology Biological Process

Gene Ontology Molecular Function

co-SMAD binding [ISO]cysteine-type endopeptidase activity [IBA]protein binding [IPI]ubiquitin thiolesterase activity [IBA]ubiquitin-specific protease activity [IBA]