BAIT

SNTB2

D16S2531E, EST25263, SNT2B2, SNT3, SNTL
syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2)
GO Process (0)
GO Function (2)
GO Component (6)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

MAST1

SAST, SAST170
microtubule associated serine/threonine kinase 1
GO Process (3)
GO Function (4)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases.

Lumeng C, Phelps S, Crawford GE, Walden PD, Barald K, Chamberlain JS

A screen for proteins that interact with beta 2-syntrophin led to the isolation of MAST205 (microtubule-associated serine/threonine kinase-205 kD) and a newly identified homologue, SAST (syntrophin-associated serine/threonine kinase). Binding studies showed that beta 2-syntrophin and MAST205/SAST associated via a PDZ-PDZ domain interaction. MAST205 colocalized with beta 2-syntrophin and utrophin at neuromuscular junctions. SAST colocalized with syntrophin in cerebral vasculature, spermatic ... [more]

Nat. Neurosci. Jul. 01, 1999; 2(7);611-7 [Pubmed: 10404183]

Throughput

  • Low Throughput

Curated By

  • BioGRID