PKN1
Gene Ontology Biological Process
Gene Ontology Molecular Function- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
VIM
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Domain-specific phosphorylation of vimentin and glial fibrillary acidic protein by PKN.
PKN is a serine/threonine protein kinase with a catalytic domain homologous to the protein kinase C family and unique N-terminal leucine zipper-like sequences. Using analyses with the yeast two-hybrid system and in vitro binding assay, we found that the regulatory domain of PKN interacted with vimentin. We then examined whether PKN would phosphorylate vimentin in vitro. Vimentin proved to be ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PKN1 VIM | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 282907 | |
| PKN1 VIM | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID