BAIT

MAD2L2

MAD2B, POLZ2, REV7, RP3-330O12.4
MAD2 mitotic arrest deficient-like 2 (yeast)
Homo sapiens
PREY

PHGDH

3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA, RP4-683H9.1
phosphoglycerate dehydrogenase
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

SHLD2/FAM35A co-operates with REV7 to coordinate DNA double-strand break repair pathway choice.

Findlay S, Heath J, Luo VM, Malina A, Morin T, Coulombe Y, Djerir B, Li Z, Samiei A, Simo-Cheyou E, Karam M, Bagci H, Rahat D, Grapton D, Lavoie EG, Dove C, Khaled H, Kuasne H, Mann KK, Klein KO, Greenwood CM, Tabach Y, Park M, Cote JF, Masson JY, Marechal A, Orthwein A

DNA double-strand breaks (DSBs) can be repaired by two major pathways: non-homologous end-joining (NHEJ) and homologous recombination (HR). DNA repair pathway choice is governed by the opposing activities of 53BP1, in complex with its effectors RIF1 and REV7, and BRCA1. However, it remains unknown how the 53BP1/RIF1/REV7 complex stimulates NHEJ and restricts HR to the S/G2 phases of the cell ... [more]

EMBO J Dec. 14, 2017; 37(18); [Pubmed: 30154076]

Throughput

  • High Throughput

Additional Notes

  • BFDR<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MAD2L2 PHGDH
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2831134

Curated By

  • BioGRID