BAIT

HLH-2

CELE_M05B5.5
Protein HLH-2
Caenorhabditis elegans
PREY

CND-1

CELE_C34E10.7, hlh-5, C34E10.7
cnd-1 encodes a basic helix-loop-helix protein that is most closely related to the vertebrate NeuroD transcription factors; during development, CND-1 is predicted to function as a transcriptional regulator whose activity is required for several aspects of motor neuron fate specification, including cell division patterns, proper spatiotemporal expression of fate-specific markers, and normal axonal morphology and pathfinding; CND-1::GFP expression begins in early embryogenesis (<20 cells) in four AB descendants and then continues in mitotically active AB-derived neuronal precursors, unidentified nuclei during gastrulation and enclosure, and postmitotic neurons in the head and ventral cord; at hatching CND-1::GFP expression is visible in ventral cord neurons, but this expression disappears completely by the end of the first larval stage; CND-1::GFP does not appear to be expressed in any non-neuronal ectodermal cells, as its expression does not overlap with that of LIN-26.
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A multiparameter network reveals extensive divergence between C. elegans bHLH transcription factors.

Grove CA, De Masi F, Barrasa MI, Newburger DE, Alkema MJ, Bulyk ML, Walhout AJ

Differences in expression, protein interactions, and DNA binding of paralogous transcription factors ("TF parameters") are thought to be important determinants of regulatory and biological specificity. However, both the extent of TF divergence and the relative contribution of individual TF parameters remain undetermined. We comprehensively identify dimerization partners, spatiotemporal expression patterns, and DNA-binding specificities for the C. elegans bHLH family of ... [more]

Cell Jul. 23, 2009; 138(2);314-27 [Pubmed: 19632181]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CND-1 HLH-2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By