PTPN11
Gene Ontology Biological Process
- ERBB signaling pathway [IDA]
- Fc-epsilon receptor signaling pathway [TAS]
- T cell costimulation [TAS]
- atrioventricular canal development [IMP]
- axon guidance [TAS]
- blood coagulation [TAS]
- brain development [IMP]
- cytokine-mediated signaling pathway [TAS]
- ephrin receptor signaling pathway [IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- face morphogenesis [IMP]
- fibroblast growth factor receptor signaling pathway [TAS]
- genitalia development [IMP]
- heart development [IMP]
- innate immune response [TAS]
- inner ear development [IMP]
- insulin receptor signaling pathway [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-tyrosine dephosphorylation [IDA, IMP]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of glucose import in response to insulin stimulus [IDA]
- regulation of cell adhesion mediated by integrin [IMP]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
- regulation of type I interferon-mediated signaling pathway [TAS]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function
IRS1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- cellular response to insulin stimulus [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glucose homeostasis [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IDA, IMP, IPI, TAS]
- insulin-like growth factor receptor signaling pathway [IPI]
- negative regulation of insulin receptor signaling pathway [ISS]
- negative regulation of insulin secretion [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [NAS]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose import in response to insulin stimulus [IDA]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of insulin receptor signaling pathway [ISS]
- positive regulation of phosphatidylinositol 3-kinase activity [ISS]
- response to insulin [IDA]
- response to peptide hormone [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The COOH-terminal tyrosine phosphorylation sites on IRS-1 bind SHP-2 and negatively regulate insulin signaling.
Activation of tyrosine kinases by numerous growth factor and cytokine receptors leads to tyrosine phosphorylation of the insulin receptor substrate (IRS)-proteins. Tyrosine-phosphorylated motifs on the IRS proteins bind to the SH2 domains in proteins that mediate downstream signals, including phosphatidylinositol 3'-kinase, GRB-2, and SHP-2. We investigated the function of the two SHP-2 binding COOH-terminal tyrosines of IRS-1 by replacing them ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PTPN11 IRS1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PTPN11 IRS1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 825212 | |
| IRS1 PTPN11 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| PTPN11 IRS1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| IRS1 PTPN11 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID