CUL-1
Gene Ontology Biological Process
- apoptotic process [IMP]
- cell migration [IMP]
- determination of adult lifespan [IMP]
- embryo development ending in birth or egg hatching [IMP]
- gonad development [IMP]
- mitotic nuclear division [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of mitotic cell cycle [IMP]
- nematode larval development [IMP]
- positive regulation of apoptotic process [IMP]
- receptor-mediated endocytosis [IMP]
- regulation of cell size [IMP]
- reproduction [IMP]
SKR-2
Gene Ontology Biological Process
- apoptotic process [IMP]
- determination of adult lifespan [IMP]
- embryo development ending in birth or egg hatching [IMP]
- establishment of mitotic spindle orientation [IMP]
- gonad development [IMP]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- meiotic chromosome segregation [IMP]
- meiotic nuclear division [IMP]
- negative regulation of cell proliferation [IMP]
- nematode larval development [IMP]
- oviposition [IMP]
- regulation of meiotic cell cycle [IMP]
- reproduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Two functionally distinct E2/E3 pairs coordinate sequential ubiquitination of a common substrate in Caenorhabditis elegans development.
Ubiquitination, the crucial posttranslational modification that regulates the eukaryotic proteome, is carried out by a trio of enzymes, known as E1 [ubiquitin (Ub)-activating enzyme], E2 (Ub-conjugating enzyme), and E3 (Ub ligase). Although most E2s can work with any of the three mechanistically distinct classes of E3s, the E2 UBCH7 is unable to function with really interesting new gene (RING)-type E3s, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SKR-2 CUL-1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| CUL-1 SKR-2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | WormBase | - | |
| SKR-2 CUL-1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.746 | BioGRID | 2583113 | |
| CUL-1 SKR-2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | WormBase | - | |
| CUL-1 SKR-2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | WormBase | - |