BAIT
ANI-1
CELE_Y49E10.19, Y49E10.19
ani-1 encodes one of three C. elegans anillins; ANI-1 activity is required in the early embryo for cortical contractile events, including polar body extrusion, membrane ruffling, and pseudocleavage; at later stages of development, ANI-1 also plays a role in cuticle formation, coordinated locomotion, vulval development, and male tail formation; in regulating cortical events, ANI-1 appears to function by positively regulating septin (UNC-59 and UNC-61) localization to the contractile ring as well as formation of septin- and NMY-2-containing cortical patches; in telophase embryos, ANI-1 localizes to the cell cortex and cleavage furrow.
GO Process (12)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
- collagen and cuticulin-based cuticle development [IMP]
- cortical cytoskeleton organization [IMP]
- embryo development ending in birth or egg hatching [IMP]
- first cell cycle pseudocleavage [IMP]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- nematode larval development [IMP]
- nematode male tail tip morphogenesis [IMP]
- polar body extrusion after meiotic divisions [IMP]
- protein localization [IMP]
- receptor-mediated endocytosis [IMP]
- septin ring organization [IMP]
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
RAN-1
CELE_K01G5.4, K01G5.4
ran-1 encodes the C. elegans Ran GTPase ortholog; ran-1 activity is essential for proper mitotic spindle formation, effective connection between centrosomes and the male pronucleus, nuclear envelope assembly after cell division, and oocyte meiotic maturation; in regulating nuclear envelope dynamics, ran-1 appears to function upstream of MEL-28 by regulating its localization to the nuclear periphery; in regulating oocyte meiotic maturation, RAN-1 interacts with VAB-1 MSP/Eph and regulates its localization to endocytic-recycling vesicles in the absence of the major sperm protein (MSP)/sperm; in dissected gonads and oocytes, RAN-1 staining is detected at the cortex.
GO Process (8)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Caenorhabditis elegans
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Sterile 20 Family Kinase and Its Co-factor CCM-3 Regulate Contractile Ring Proteins on Germline Intercellular Bridges.
Germ cells in most animals are connected by intercellular bridges, actin-based rings that form stable cytoplasmic connections between cells promoting communication and coordination [1]. Moreover, these connections are required for fertility [1, 2]. Intercellular bridges are proposed to arise from stabilization of the cytokinetic ring during incomplete cytokinesis [1]. Paradoxically, proteins that promote closure of cytokinetic rings are enriched on ... [more]
Curr Biol Mar. 20, 2017; 27(6);860-867 [Pubmed: 28285996]
Throughput
- High Throughput