BAIT

CSPG5

NGC
chondroitin sulfate proteoglycan 5 (neuroglycan C)
Homo sapiens
PREY

GOPC

CAL, FIG, GOPC1, PIST, dJ94G16.2
golgi-associated PDZ and coiled-coil motif containing
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CALEB/NGC interacts with the Golgi-associated protein PIST.

Hassel B, Schreff M, Stube EM, Blaich U, Schumacher S

CALEB/NGC is a neural member of the epidermal growth factor protein family expressed in axon and synapse-rich areas of the nervous system and shown to be important for neurite formation. It can bind to the extracellular matrix proteins tenascin-R and tenascin-C. Here we show that CALEB/NGC interacts with the Golgi-associated protein PIST. PIST was originally described as an interaction partner ... [more]

J. Biol. Chem. Oct. 10, 2003; 278(41);40136-43 [Pubmed: 12885772]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GOPC CSPG5
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID