LAG-1
Gene Ontology Biological Process
- Notch signaling pathway [IPI]
- cell division [IMP]
- cell fate specification [IMP]
- collagen and cuticulin-based cuticle development [IMP]
- dauer exit [IGI]
- embryo development ending in birth or egg hatching [IMP]
- gene expression [IMP]
- germ-line stem cell division [IMP]
- hermaphrodite genitalia development [IMP]
- molting cycle, collagen and cuticulin-based cuticle [IMP]
- nematode larval development [IMP]
- oviposition [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of vulval development [IMP]
- regulation of cell fate specification [IMP]
- reproduction [IMP]
- vulval development [IMP]
Gene Ontology Molecular Function
GLP-1
Gene Ontology Biological Process
- body morphogenesis [IMP]
- cell fate specification [IGI]
- determination of adult lifespan [IMP]
- embryo development ending in birth or egg hatching [IMP]
- embryonic pattern specification [IGI, IMP]
- maintenance of dauer [IGI]
- nematode larval development [IGI, IMP]
- pharyngeal muscle development [IGI, IMP]
- regulation of cell proliferation [IMP]
- regulation of meiosis [IMP]
- reproduction [IMP]
Gene Ontology Cellular Component
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Roles of the RAM and ANK domains in signaling by the C. elegans GLP-1 receptor.
In Caenorhabditis elegans, the GLP-1 receptor acts with a downstream transcriptional regulator, LAG-1, to mediate intercellular signaling. GLP-1 and LAG-1 are homologs of Drosophila Notch and Su(H) respectively. Here, we investigate the functions of two regions of the GLP-1 intracellular domain: the ANK repeat domain, which includes six cdc10/ankyrin repeats plus flanking amino acids, and the RAM domain, which spans ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
LAG-1 GLP-1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | WormBase | - | |
LAG-1 GLP-1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | WormBase | - | |
GLP-1 LAG-1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | 4 | BioGRID | 466722 | |
LAG-1 GLP-1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
LAG-1 GLP-1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | WormBase | - | |
LAG-1 GLP-1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | WormBase | - |