An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity.

Kim JJ, Lee SY, Gong F, Battenhouse AM, Boutz DR, Bashyal A, Refvik ST, Chiang CM, Xhemalce B, Paull TT, Brodbelt JS, Marcotte EM, Miller KM

Bromodomain proteins (BRD) are key chromatin regulators of genome function and stability as well as therapeutic targets in cancer. Here, we systematically delineate the contribution of human BRD proteins for genome stability and DNA double-strand break (DSB) repair using several cell-based assays and proteomic interaction network analysis. Applying these approaches, we identify 24 of the 42 BRD proteins as promoters ... [more]

Genes Dev Dec. 01, 2018; 33(23-24);1751-1774 [Pubmed: 31753913]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

TRIM24

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]estrogen response element binding [IDA]lysine-acetylated histone binding [IDA]methylated histone binding [IDA]p53 binding [IPI]protein binding [IPI]receptor binding [TAS]transcription coactivator activity [IDA]ubiquitin-protein transferase activity [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

PSD