BAIT

AR

AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, RP11-383C12.1
androgen receptor
GO Process (23)
GO Function (16)
GO Component (5)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.

Leung KK, Hause RJ, Barkinge JL, Ciaccio MF, Chuu CP, Jones RB

Many human diseases are associated with aberrant regulation of phosphoprotein signaling networks. Src homology 2 (SH2) domains represent the major class of protein domains in metazoans that interact with proteins phosphorylated on the amino acid residue tyrosine. Although current SH2 domain prediction algorithms perform well at predicting the sequences of phosphorylated peptides that are likely to result in the highest ... [more]

Mol. Cell Proteomics Jul. 01, 2014; 13(7);1705-23 [Pubmed: 24728074]

Quantitative Score

  • 3.56 [KD]

Throughput

  • High Throughput

Additional Notes

  • interaction assayed using fluorescence polarization (FP) measurements using one or more phosphopeptides derived from the bait protein and all or a portion of the purified prey protein
  • this results in one or more KD values measured in micromolar (3.56,5.27,8.98,10.8,19.19) of which the minimum value representing the highest affinity interaction is reported in the HTP score

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AR YES1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID