BAIT

KIT

C-Kit, CD117, PBT, SCFR
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
GO Process (53)
GO Function (5)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

SHC1

SHC, SHCA, RP11-307C12.1
SHC (Src homology 2 domain containing) transforming protein 1
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.

Leung KK, Hause RJ, Barkinge JL, Ciaccio MF, Chuu CP, Jones RB

Many human diseases are associated with aberrant regulation of phosphoprotein signaling networks. Src homology 2 (SH2) domains represent the major class of protein domains in metazoans that interact with proteins phosphorylated on the amino acid residue tyrosine. Although current SH2 domain prediction algorithms perform well at predicting the sequences of phosphorylated peptides that are likely to result in the highest ... [more]

Mol. Cell Proteomics Jul. 01, 2014; 13(7);1705-23 [Pubmed: 24728074]

Quantitative Score

  • 0.37 [KD]

Throughput

  • High Throughput

Additional Notes

  • interaction assayed using fluorescence polarization (FP) measurements using one or more phosphopeptides derived from the bait protein and all or a portion of the purified prey protein
  • this results in one or more KD values measured in micromolar (0.37,0.41,1.73,4.63,6.94,11.03,12.98,14.37) of which the minimum value representing the highest affinity interaction is reported in the HTP score

Curated By

  • BioGRID