BAIT

TRIM54

MURF, MURF-3, RNF30, muRF3
tripartite motif containing 54
GO Process (3)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy.

Nowak M, Suenkel B, Porras P, Migotti R, Schmidt F, Kny M, Zhu X, Wanker EE, Dittmar G, Fielitz J, Sommer T

The muscle-specific RING-finger protein MuRF1 (also known as TRIM63) constitutes a bona fide ubiquitin ligase that routes proteins like several different myosin heavy chain proteins (MyHC) to proteasomal degradation during muscle atrophy. In two unbiased screens, we identified DCAF8 as a new MuRF1-binding partner. MuRF1 physically interacts with DCAF8 and both proteins localize to overlapping structures in muscle cells. Importantly, ... [more]

J. Cell. Sci. Dec. 06, 2018; 132(17); [Pubmed: 31391242]

Quantitative Score

  • 0.25 [Custom Score]

Throughput

  • High Throughput

Additional Notes

  • for each interaction detected, a custom score was provided based on a composite measure of plate growth and reporter expression, with a value between 0 and 1, with 1 being the strongest apparent interaction
  • one or more bait construct variants were found to interact with the prey gene in a yeast two hybrid assay in one or more samples each
  • the maximum of potentially several such scores is reported here

Curated By

  • BioGRID