BAIT
TRIM54
MURF, MURF-3, RNF30, muRF3
tripartite motif containing 54
GO Process (3)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRMT5
HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs
protein arginine methyltransferase 5
GO Process (13)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cell proliferation [TAS]
- circadian regulation of gene expression [ISS]
- endothelial cell activation [IMP]
- gene expression [TAS]
- histone H4-R3 methylation [ISS, NAS]
- ncRNA metabolic process [TAS]
- peptidyl-arginine N-methylation [IDA]
- peptidyl-arginine methylation [IMP]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IMP]
- regulation of mitosis [TAS]
- regulation of transcription, DNA-templated [IBA]
- spliceosomal snRNP assembly [IMP, TAS]
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy.
The muscle-specific RING-finger protein MuRF1 (also known as TRIM63) constitutes a bona fide ubiquitin ligase that routes proteins like several different myosin heavy chain proteins (MyHC) to proteasomal degradation during muscle atrophy. In two unbiased screens, we identified DCAF8 as a new MuRF1-binding partner. MuRF1 physically interacts with DCAF8 and both proteins localize to overlapping structures in muscle cells. Importantly, ... [more]
J. Cell. Sci. Dec. 06, 2018; 132(17); [Pubmed: 31391242]
Quantitative Score
- 0.416666667 [Custom Score]
Throughput
- High Throughput
Additional Notes
- for each interaction detected, a custom score was provided based on a composite measure of plate growth and reporter expression, with a value between 0 and 1, with 1 being the strongest apparent interaction
- one or more bait construct variants were found to interact with the prey gene in a yeast two hybrid assay in one or more samples each
- the maximum of potentially several such scores is reported here
Curated By
- BioGRID