PREY
CAMK2A
CAMKA
calcium/calmodulin-dependent protein kinase II alpha
GO Process (15)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISS]
- calcium ion transport [ISS]
- cytokine-mediated signaling pathway [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- peptidyl-serine phosphorylation [ISS]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of calcium ion transport [ISS]
- positive regulation of cardiac muscle cell apoptotic process [ISS]
- protein autophosphorylation [ISS]
- protein phosphorylation [IDA]
- regulation of mitochondrial membrane permeability involved in apoptotic process [ISS]
- regulation of neuronal synaptic plasticity [ISS]
- regulation of neurotransmitter secretion [ISS]
- response to ischemia [ISS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy.
The muscle-specific RING-finger protein MuRF1 (also known as TRIM63) constitutes a bona fide ubiquitin ligase that routes proteins like several different myosin heavy chain proteins (MyHC) to proteasomal degradation during muscle atrophy. In two unbiased screens, we identified DCAF8 as a new MuRF1-binding partner. MuRF1 physically interacts with DCAF8 and both proteins localize to overlapping structures in muscle cells. Importantly, ... [more]
J. Cell. Sci. Dec. 06, 2018; 132(17); [Pubmed: 31391242]
Quantitative Score
- 0.33 [Custom Score]
Throughput
- High Throughput
Additional Notes
- for each interaction detected, a custom score was provided based on a composite measure of plate growth and reporter expression, with a value between 0 and 1, with 1 being the strongest apparent interaction
- one or more bait construct variants were found to interact with the prey gene in a yeast two hybrid assay in one or more samples each
- the maximum of potentially several such scores is reported here
Curated By
- BioGRID