Publication

DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy.

Nowak M, Suenkel B, Porras P, Migotti R, Schmidt F, Kny M, Zhu X, Wanker EE, Dittmar G, Fielitz J, Sommer T

The muscle-specific RING-finger protein MuRF1 (also known as TRIM63) constitutes a bona fide ubiquitin ligase that routes proteins like several different myosin heavy chain proteins (MyHC) to proteasomal degradation during muscle atrophy. In two unbiased screens, we identified DCAF8 as a new MuRF1-binding partner. MuRF1 physically interacts with DCAF8 and both proteins localize to overlapping structures in muscle cells. Importantly, ... [more]

Quantitative Score

• 0.33 [Custom Score]

Throughput

• High Throughput

Additional Notes

• for each interaction detected, a custom score was provided based on a composite measure of plate growth and reporter expression, with a value between 0 and 1, with 1 being the strongest apparent interaction
• one or more bait construct variants were found to interact with the prey gene in a yeast two hybrid assay in one or more samples each
• the maximum of potentially several such scores is reported here

Curated By

• BioGRID