BAIT

TRIM63

IRF, MURF1, MURF2, RNF28, SMRZ
tripartite motif containing 63, E3 ubiquitin protein ligase
UPS Project
GO Process (2)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SYNCRIP

GRY-RBP, GRYRBP, HNRNPQ, HNRPQ1, NSAP1, PP68, hnRNP-Q, RP1-3J17.2
synaptotagmin binding, cytoplasmic RNA interacting protein
Autism spectrum disorder (ASD) Project
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy.

Nowak M, Suenkel B, Porras P, Migotti R, Schmidt F, Kny M, Zhu X, Wanker EE, Dittmar G, Fielitz J, Sommer T

The muscle-specific RING-finger protein MuRF1 (also known as TRIM63) constitutes a bona fide ubiquitin ligase that routes proteins like several different myosin heavy chain proteins (MyHC) to proteasomal degradation during muscle atrophy. In two unbiased screens, we identified DCAF8 as a new MuRF1-binding partner. MuRF1 physically interacts with DCAF8 and both proteins localize to overlapping structures in muscle cells. Importantly, ... [more]

J. Cell. Sci. Dec. 06, 2018; 132(17); [Pubmed: 31391242]

Quantitative Score

  • 0.53 [Custom Score]

Throughput

  • High Throughput

Additional Notes

  • for each interaction detected, a custom score was provided based on a composite measure of plate growth and reporter expression, with a value between 0 and 1, with 1 being the strongest apparent interaction
  • one or more bait construct variants were found to interact with the prey gene in a yeast two hybrid assay in one or more samples each
  • the maximum of potentially several such scores is reported here

Curated By

  • BioGRID