BAIT
PYHIN1
IFIX, RP11-520H16.1
pyrin and HIN domain family, member 1
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
UCHL1
HEL-117, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, Uch-L1
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
GO Process (3)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The functional interactome of PYHIN immune regulators reveals IFIX is a sensor of viral DNA.
The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are critical regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins and define a function in sensing of viral DNA for the previously uncharacterized IFIX protein. By designing a cell-based inducible ... [more]
Unknown Feb. 09, 2015; 11(1);787 [Pubmed: 25665578]
Quantitative Score
- 0.864 [Saint Score]
Throughput
- High Throughput
Additional Notes
- note, however, that inclusion of interactors in the article was dependent on more complex criteria than that single averaged SAINT score
- the indicated score for the interactor/prey protein is the average of SAINT scores for that interactor from affinity purification experiments using both N- and C-terminally tagged constructs of the indicated bait protein
Curated By
- BioGRID