BAIT
FARP1
CDEP, FARP1-IT1, PLEKHC2, PPP1R75, RP11-111L24.1
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
GO Process (5)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
NOC2L
NET15, NET7, NIR, PPP1R112
nucleolar complex associated 2 homolog (S. cerevisiae)
GO Process (7)
GO Function (7)
GO Component (3)
Gene Ontology Biological Process
- cellular response to UV [IDA]
- chromatin assembly [IDA]
- negative regulation of B cell apoptotic process [IMP]
- negative regulation of histone acetylation [IDA]
- negative regulation of intrinsic apoptotic signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- nucleolus to nucleoplasm transport [IDA]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.
Rho GTPases are central regulators of the cytoskeleton and, in humans, are controlled by 145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs). How Rho signalling patterns are established in dynamic cell spaces to control cellular morphogenesis is unclear. Through a family-wide characterization of substrate specificities, interactomes and localization, we reveal at the systems level how RhoGEFs and ... [more]
Nat. Cell Biol. Dec. 01, 2019; 22(4);498-511 [Pubmed: 32203420]
Quantitative Score
- 6.184931149 [CompPASS Z-score]
Throughput
- High Throughput
Additional Notes
- prey proteins were classified into confidence-level categories of gold (highest confidence), silver or bronze based on various criteria
- tagged bait proteins were affinity purified and associated proteins identified by mass spectrometry
- the score provided is the CompPASS Z-score
- this interaction was classified by the authors as being in the gold list
Curated By
- BioGRID