An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.

Mueller PM, Rademacher J, Bagshaw RD, Wortmann C, Barth C, van Unen J, Alp KM, Giudice G, Eccles RL, Heinrich LE, Pascual-Vargas P, Sanchez-Castro M, Brandenburg L, Mbamalu G, Tucholska M, Spatt L, Czajkowski MT, Welke RW, Zhang S, Nguyen V, Rrustemi T, Trnka P, Freitag K, Larsen B, Popp O, Mertins P, Gingras AC, Roth FP, Colwill K, Bakal C, Pertz O, Pawson T, Petsalaki E, Rocks O

Rho GTPases are central regulators of the cytoskeleton and, in humans, are controlled by 145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs). How Rho signalling patterns are established in dynamic cell spaces to control cellular morphogenesis is unclear. Through a family-wide characterization of substrate specificities, interactomes and localization, we reveal at the systems level how RhoGEFs and ... [more]

Nat. Cell Biol. Dec. 01, 2019; 22(4);498-511 [Pubmed: 32203420]

Quantitative Score

3.872983346 [CompPASS Z-score]

Throughput

High Throughput

Additional Notes

prey proteins were classified into confidence-level categories of gold (highest confidence), silver or bronze based on various criteria
tagged bait proteins were affinity purified and associated proteins identified by mass spectrometry
the score provided is the CompPASS Z-score
this interaction was classified by the authors as being in the gold list

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ITSN1 EPS15	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sengar AS (1999)	Low	-	BioGRID	722547
ITSN1 EPS15	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.3099	BioGRID	1261839
ITSN1 EPS15	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wong KA (2012)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ITSN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [NAS]guanyl-nucleotide exchange factor activity [NAS]proline-rich region binding [IPI]protein binding [IPI]protein complex scaffold [IDA]

Gene Ontology Cellular Component

EPS15