BAIT

SYDE1

7h3
synapse defective 1, Rho GTPase, homolog 1 (C. elegans)
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

APBB1

FE65, MGC:9072, RIR
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Alzheimer's Disease Project
Autism spectrum disorder (ASD) Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.

Mueller PM, Rademacher J, Bagshaw RD, Wortmann C, Barth C, van Unen J, Alp KM, Giudice G, Eccles RL, Heinrich LE, Pascual-Vargas P, Sanchez-Castro M, Brandenburg L, Mbamalu G, Tucholska M, Spatt L, Czajkowski MT, Welke RW, Zhang S, Nguyen V, Rrustemi T, Trnka P, Freitag K, Larsen B, Popp O, Mertins P, Gingras AC, Roth FP, Colwill K, Bakal C, Pertz O, Pawson T, Petsalaki E, Rocks O

Rho GTPases are central regulators of the cytoskeleton and, in humans, are controlled by 145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs). How Rho signalling patterns are established in dynamic cell spaces to control cellular morphogenesis is unclear. Through a family-wide characterization of substrate specificities, interactomes and localization, we reveal at the systems level how RhoGEFs and ... [more]

Nat. Cell Biol. Dec. 01, 2019; 22(4);498-511 [Pubmed: 32203420]

Quantitative Score

  • 7.479424306 [CompPASS Z-score]

Throughput

  • High Throughput

Additional Notes

  • prey proteins were classified into confidence-level categories of gold (highest confidence), silver or bronze based on various criteria
  • tagged bait proteins were affinity purified and associated proteins identified by mass spectrometry
  • the score provided is the CompPASS Z-score
  • this interaction was classified by the authors as being in the gold list

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
APBB1 SYDE1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2222216
SYDE1 APBB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9895BioGRID
3189834
APBB1 SYDE1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3038953

Curated By

  • BioGRID