BAIT
ALS2
ALS2CR6, ALSJ, IAHSP, PLSJ
amyotrophic lateral sclerosis 2 (juvenile)
GO Process (9)
GO Function (8)
GO Component (9)
Gene Ontology Biological Process
- endosome organization [IGI, NAS]
- neuron projection morphogenesis [IDA]
- positive regulation of Rab GTPase activity [IDA]
- positive regulation of Rac GTPase activity [IDA]
- positive regulation of Rac protein signal transduction [IC]
- positive regulation of Ran GTPase activity [NAS]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of endosome size [IEP]
Gene Ontology Molecular Function- Rab GTPase binding [IDA, NAS]
- Rab guanyl-nucleotide exchange factor activity [IDA]
- Rac guanyl-nucleotide exchange factor activity [IDA]
- Ran guanyl-nucleotide exchange factor activity [NAS]
- guanyl-nucleotide exchange factor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein serine/threonine kinase activator activity [IDA]
- Rab GTPase binding [IDA, NAS]
- Rab guanyl-nucleotide exchange factor activity [IDA]
- Rac guanyl-nucleotide exchange factor activity [IDA]
- Ran guanyl-nucleotide exchange factor activity [NAS]
- guanyl-nucleotide exchange factor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
CHN1
ARHGAP2, CHN, DURS2, NC, RHOGAP2
chimerin 1
GO Process (6)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.
Rho GTPases are central regulators of the cytoskeleton and, in humans, are controlled by 145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs). How Rho signalling patterns are established in dynamic cell spaces to control cellular morphogenesis is unclear. Through a family-wide characterization of substrate specificities, interactomes and localization, we reveal at the systems level how RhoGEFs and ... [more]
Nat. Cell Biol. Dec. 01, 2019; 22(4);498-511 [Pubmed: 32203420]
Quantitative Score
- 1.435860077 [CompPASS Z-score]
Throughput
- High Throughput
Additional Notes
- prey proteins were classified into confidence-level categories of gold (highest confidence), silver or bronze based on various criteria
- tagged bait proteins were affinity purified and associated proteins identified by mass spectrometry
- the score provided is the CompPASS Z-score
- this interaction was classified by the authors as being in the bronze list
Curated By
- BioGRID