ERBB3
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Schwann cell differentiation [ISS]
- cranial nerve development [ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- extrinsic apoptotic signaling pathway in absence of ligand [IMP]
- fibroblast growth factor receptor signaling pathway [TAS]
- heart development [ISS]
- innate immune response [TAS]
- negative regulation of cell adhesion [IDA]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of secretion [IDA]
- negative regulation of signal transduction [IDA]
- neuron apoptotic process [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- peripheral nervous system development [ISS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of phosphatidylinositol 3-kinase signaling [TAS]
- positive regulation of protein tyrosine kinase activity [IMP]
- regulation of cell proliferation [IDA]
- signal transduction [IDA]
- transmembrane receptor protein tyrosine kinase signaling pathway [ISS]
- wound healing [NAS]
Gene Ontology Molecular Function- growth factor binding [IPI, ISS]
- identical protein binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- protein homodimerization activity [NAS]
- protein tyrosine kinase activator activity [IDA]
- protein tyrosine kinase activity [IDA]
- transmembrane signaling receptor activity [ISS]
- growth factor binding [IPI, ISS]
- identical protein binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- protein homodimerization activity [NAS]
- protein tyrosine kinase activator activity [IDA]
- protein tyrosine kinase activity [IDA]
- transmembrane signaling receptor activity [ISS]
Gene Ontology Cellular Component
SHC1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [IDA]
- Ras protein signal transduction [TAS]
- activation of MAPK activity [IDA]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- blood coagulation [TAS]
- cellular protein metabolic process [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IBA, ISS, TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-tyrosine phosphorylation [TAS]
- platelet activation [TAS]
- positive regulation of DNA replication [ISS]
- positive regulation of cell proliferation [NAS]
- regulation of epidermal growth factor-activated receptor activity [TAS]
Gene Ontology Molecular Function- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [ISS]
- insulin receptor binding [IPI]
- insulin-like growth factor receptor binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- phospholipid binding [TAS]
- protein binding [IPI]
- protein kinase binding [IBA]
- protein tyrosine kinase activity [TAS]
- transmembrane receptor protein tyrosine kinase adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [ISS]
- insulin receptor binding [IPI]
- insulin-like growth factor receptor binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- phospholipid binding [TAS]
- protein binding [IPI]
- protein kinase binding [IBA]
- protein tyrosine kinase activity [TAS]
- transmembrane receptor protein tyrosine kinase adaptor activity [TAS]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Comprehensive binary interaction mapping of SH2 domains via fluorescence polarization reveals novel functional diversification of ErbB receptors.
First-generation interaction maps of Src homology 2 (SH2) domains with receptor tyrosine kinase (RTK) phosphosites have previously been generated using protein microarray (PM) technologies. Here, we developed a large-scale fluorescence polarization (FP) methodology that was able to characterize interactions between SH2 domains and ErbB receptor phosphosites with higher fidelity and sensitivity than was previously achieved with PMs. We used the ... [more]
Quantitative Score
- 0.14 [KD]
Throughput
- High Throughput
Additional Notes
- interaction assayed using fluorescence polarization (FP) measurements using one or more phosphopeptides derived from the bait protein and all or a portion of the purified prey protein
- this results in one or more KD values measured in micromolar (0.14,2.15,2.93,5.265,5.74,6.18,6.59,8.94,9.66,10.1,18.23) of which the minimum value representing the highest affinity interaction is reported in the HTP score
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ERBB3 SHC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
ERBB3 SHC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
ERBB3 SHC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
SHC1 ERBB3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
ERBB3 SHC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
ERBB3 SHC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
ERBB3 SHC1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | High | - | BioGRID | 833496 | |
ERBB3 SHC1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 0.01 | BioGRID | 3508740 |
Curated By
- BioGRID