ERBB4
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- cardiac muscle tissue regeneration [ISS]
- cell migration [IDA]
- cell proliferation [TAS]
- central nervous system morphogenesis [ISS]
- embryonic pattern specification [ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- heart development [ISS]
- innate immune response [TAS]
- lactation [IMP]
- mammary gland alveolus development [ISS]
- mammary gland epithelial cell differentiation [ISS]
- mitochondrial fragmentation involved in apoptotic process [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- nervous system development [ISS]
- neural crest cell migration [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- olfactory bulb interneuron differentiation [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of STAT protein import into nucleus [IMP]
- positive regulation of cardiac muscle cell proliferation [ISS]
- positive regulation of cell proliferation [IMP]
- positive regulation of phosphatidylinositol 3-kinase activity [IDA]
- positive regulation of protein phosphorylation [TAS]
- positive regulation of transcription, DNA-templated [IMP]
- positive regulation of tyrosine phosphorylation of Stat5 protein [IMP]
- protein autophosphorylation [IDA]
- regulation of cell migration [ISS]
- signal transduction [IDA]
- transmembrane receptor protein tyrosine kinase signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GRB2
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- Ras protein signal transduction [TAS]
- T cell costimulation [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell-cell signaling [TAS]
- cellular response to ionizing radiation [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IPI, TAS]
- leukocyte migration [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- platelet activation [TAS]
- positive regulation of reactive oxygen species metabolic process [IMP]
- receptor internalization [IMP]
- signal transduction in response to DNA damage [IMP]
Gene Ontology Molecular Function- SH3 domain binding [IDA]
- SH3/SH2 adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [IPI]
- identical protein binding [IPI]
- insulin receptor substrate binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- SH3 domain binding [IDA]
- SH3/SH2 adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [IPI]
- identical protein binding [IPI]
- insulin receptor substrate binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Comprehensive binary interaction mapping of SH2 domains via fluorescence polarization reveals novel functional diversification of ErbB receptors.
First-generation interaction maps of Src homology 2 (SH2) domains with receptor tyrosine kinase (RTK) phosphosites have previously been generated using protein microarray (PM) technologies. Here, we developed a large-scale fluorescence polarization (FP) methodology that was able to characterize interactions between SH2 domains and ErbB receptor phosphosites with higher fidelity and sensitivity than was previously achieved with PMs. We used the ... [more]
Quantitative Score
- 1.46 [KD]
Throughput
- High Throughput
Additional Notes
- interaction assayed using fluorescence polarization (FP) measurements using one or more phosphopeptides derived from the bait protein and all or a portion of the purified prey protein
- this results in one or more KD values measured in micromolar (1.46,2.463333333) of which the minimum value representing the highest affinity interaction is reported in the HTP score
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ERBB4 GRB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| ERBB4 GRB2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID