BAIT
C21ORF33
ES1, GT335, HES1, KNPH, KNPI
chromosome 21 open reading frame 33
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
LONP1
LON, LONP, LonHS, PIM1, PRSS15, hLON
lon peptidase 1, mitochondrial
GO Process (8)
GO Function (12)
GO Component (5)
Gene Ontology Biological Process
- cellular response to oxidative stress [IC, IDA]
- mitochondrial DNA metabolic process [NAS]
- mitochondrial genome maintenance [NAS]
- mitochondrion organization [IMP]
- oxidation-dependent protein catabolic process [IMP]
- protein homooligomerization [IDA]
- proteolysis involved in cellular protein catabolic process [IDA]
- response to hypoxia [IEP]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA]
- ATP-dependent peptidase activity [IDA]
- DNA polymerase binding [IPI]
- G-quadruplex DNA binding [IDA]
- mitochondrial heavy strand promoter anti-sense binding [IDA]
- mitochondrial heavy strand promoter sense binding [IDA]
- mitochondrial light strand promoter anti-sense binding [IDA]
- mitochondrial light strand promoter sense binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- single-stranded RNA binding [IDA]
- ADP binding [IDA]
- ATP binding [IDA]
- ATP-dependent peptidase activity [IDA]
- DNA polymerase binding [IPI]
- G-quadruplex DNA binding [IDA]
- mitochondrial heavy strand promoter anti-sense binding [IDA]
- mitochondrial heavy strand promoter sense binding [IDA]
- mitochondrial light strand promoter anti-sense binding [IDA]
- mitochondrial light strand promoter sense binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- single-stranded RNA binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A High-Density Human Mitochondrial Proximity Interaction Network.
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]
Cell Metab. Sep. 01, 2020; 32(3);479-497.e9 [Pubmed: 32877691]
Quantitative Score
- 0.99 [Saint Score]
Throughput
- High Throughput
Additional Notes
- interaction assayed using BioID
- interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
- the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown
Curated By
- BioGRID