BAIT

FKBP8

FKBP38, FKBPr38
FK506 binding protein 8, 38kDa
Homo sapiens
PREY

PDCL

PhLP, RP11-465F21.2
phosducin-like
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A High-Density Human Mitochondrial Proximity Interaction Network.

Antonicka H, Lin ZY, Janer A, Aaltonen MJ, Weraarpachai W, Gingras AC, Shoubridge EA

We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]

Cell Metab. Sep. 01, 2020; 32(3);479-497.e9 [Pubmed: 32877691]

Quantitative Score

  • 1.0 [Saint Score]

Throughput

  • High Throughput

Additional Notes

  • interaction assayed using BioID
  • interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
  • the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FKBP8 PDCL
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Low-BioGRID
-
FKBP8 PDCL
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3356262
FKBP8 PDCL
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID