BAIT

RPD3

MOF6, REC3, SDI2, SDS6, histone deacetylase RPD3, L000001696, L000001603, YNL330C
Histone deacetylase, component of both the Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the arginine methyltransferase, Hmt1p
GO Process (19)
GO Function (3)
GO Component (6)
Saccharomyces cerevisiae (S288c)
PREY

VPS71

SWC6, YML041C
Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation.

Lin YY, Qi Y, Lu JY, Pan X, Yuan DS, Zhao Y, Bader JS, Boeke JD

Histone acetylation and deacetylation are among the principal mechanisms by which chromatin is regulated during transcription, DNA silencing, and DNA repair. We analyzed patterns of genetic interactions uncovered during comprehensive genome-wide analyses in yeast to probe how histone acetyltransferase (HAT) and histone deacetylase (HDAC) protein complexes interact. The genetic interaction data unveil an underappreciated role of HDACs in maintaining cellular ... [more]

Genes Dev. Aug. 01, 2008; 22(15);2062-74 [Pubmed: 18676811]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • High Throughput: dSLAM analysis was performed to determine genome-wide genetic interaction profiles of 38 query genes involved in histone (de)acetylation.
  • Low Throughput: Genetic interactions identified using dSLAM were validated by tetrad dissection and/or random spore analysis.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPD3 VPS71
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.4112BioGRID
217089
RPD3 VPS71
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.353BioGRID
407323
RPD3 VPS71
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4791BioGRID
2176173
VPS71 RPD3
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
517337

Curated By

  • BioGRID