BAIT

RTT109

KIM2, REM50, H3 histone acetyltransferase RTT109, KAT11, L000003932, YLL002W
Histone acetyltransferase; critical for cell survival in the presence of DNA damage during S phase; prevents hyper-amplification of rDNA; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p
Saccharomyces cerevisiae (S288c)
PREY

PPR1

L000001475, YLR014C
Zinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation.

Lin YY, Qi Y, Lu JY, Pan X, Yuan DS, Zhao Y, Bader JS, Boeke JD

Histone acetylation and deacetylation are among the principal mechanisms by which chromatin is regulated during transcription, DNA silencing, and DNA repair. We analyzed patterns of genetic interactions uncovered during comprehensive genome-wide analyses in yeast to probe how histone acetyltransferase (HAT) and histone deacetylase (HDAC) protein complexes interact. The genetic interaction data unveil an underappreciated role of HDACs in maintaining cellular ... [more]

Genes Dev. Aug. 01, 2008; 22(15);2062-74 [Pubmed: 18676811]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • High Throughput: dSLAM analysis was performed to determine genome-wide genetic interaction profiles of 38 query genes involved in histone (de)acetylation.
  • Low Throughput: Genetic interactions identified using dSLAM were validated by tetrad dissection and/or random spore analysis.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RTT109 PPR1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
300134

Curated By

  • BioGRID