BAIT
SAMM50
OMP85, SAM50, TOB55, TRG-3, YNL026W, CGI-51
SAMM50 sorting and assembly machinery component
GO Process (3)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HDAC3
HD3, RPD3, RPD3-2
histone deacetylase 3
GO Process (19)
GO Function (9)
GO Component (8)
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- cellular lipid metabolic process [TAS]
- cellular response to fluid shear stress [IDA]
- chromatin modification [TAS]
- negative regulation of JNK cascade [IMP]
- negative regulation of apoptotic process [TAS]
- negative regulation of cell cycle [TAS]
- negative regulation of myotube differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- negative regulation of transcription, DNA-templated [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of TOR signaling [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of transcription factor import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein deacetylation [IDA]
- regulation of protein stability [IDA]
- small molecule metabolic process [TAS]
- spindle assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A High-Density Human Mitochondrial Proximity Interaction Network.
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]
Cell Metab. Sep. 01, 2020; 32(3);479-497.e9 [Pubmed: 32877691]
Quantitative Score
- 0.97 [Saint Score]
Throughput
- High Throughput
Additional Notes
- interaction assayed using BioID
- interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
- the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown
Curated By
- BioGRID