BAIT
SLC25A46
solute carrier family 25, member 46
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
EIF2AK3
PEK, PERK, WRS
eukaryotic translation initiation factor 2-alpha kinase 3
GO Process (26)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- ER overload response [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- angiogenesis [IMP]
- bone mineralization [ISS]
- calcium-mediated signaling [ISS]
- cellular protein metabolic process [TAS]
- cellular response to glucose starvation [IMP]
- chondrocyte development [ISS]
- endocrine pancreas development [IMP]
- endoplasmic reticulum organization [ISS]
- endoplasmic reticulum unfolded protein response [IDA, TAS]
- insulin secretion [ISS]
- insulin-like growth factor receptor signaling pathway [ISS]
- negative regulation of myelination [ISS]
- negative regulation of translation [TAS]
- negative regulation of translational initiation in response to stress [TAS]
- ossification [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of transcription from RNA polymerase I promoter [IMP]
- positive regulation vascular endothelial growth factor production [IMP]
- protein autophosphorylation [IDA, IMP]
- protein homooligomerization [IMP]
- protein phosphorylation [ISS]
- response to endoplasmic reticulum stress [IMP]
- skeletal system development [ISS]
Gene Ontology Molecular Function
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A High-Density Human Mitochondrial Proximity Interaction Network.
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]
Cell Metab. Sep. 01, 2020; 32(3);479-497.e9 [Pubmed: 32877691]
Quantitative Score
- 1.0 [Saint Score]
Throughput
- High Throughput
Additional Notes
- interaction assayed using BioID
- interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
- the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown
Curated By
- BioGRID