An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Hop modulates Hsp70/Hsp90 interactions in protein folding.

Johnson BD, Schumacher RJ, Ross ED, Toft DO

Hop is a 60-kDa protein characterized by its ability to bind the two chaperones, hsp70 and hsp90. We have tested the function of Hop using an assay for the refolding of denatured firefly luciferase. We show that Hop is involved in the process of refolding thermally denatured firefly luciferase in rabbit reticulocyte lysate. Hop also stimulates refolding by hsp70 and ... [more]

J. Biol. Chem. Feb. 06, 1998; 273(6);3679-86 [Pubmed: 9452498]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STIP1 HSPA4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bhattacharya K (2020)	High	-	BioGRID	-
HSPA4 STIP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3358568
STIP1 HSPA4	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.8629	BioGRID	1263342
HSPA4 STIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Brinker A (2002)	Low	-	BioGRID	-
STIP1 HSPA4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Graumann K (2000)	Low	-	BioGRID	-
STIP1 HSPA4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Horibe T (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

STIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HSPA4