CCL2
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger [TAS]
- JAK-STAT cascade [TAS]
- MAPK cascade [IMP]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- angiogenesis [TAS]
- astrocyte cell migration [IDA]
- cell adhesion [TAS]
- cell surface receptor signaling pathway [TAS]
- cellular homeostasis [TAS]
- cellular protein metabolic process [TAS]
- cellular response to fibroblast growth factor stimulus [IEP]
- cellular response to interferon-gamma [IEP, ISS]
- cellular response to interleukin-1 [IEP, ISS]
- cellular response to lipopolysaccharide [ISS]
- cellular response to organic cyclic compound [IDA]
- cellular response to tumor necrosis factor [IEP, ISS]
- chemotaxis [TAS]
- cytokine-mediated signaling pathway [IDA]
- cytoskeleton organization [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- helper T cell extravasation [ISS]
- humoral immune response [TAS]
- inflammatory response [IDA]
- lipopolysaccharide-mediated signaling pathway [IDA]
- macrophage chemotaxis [IDA]
- monocyte chemotaxis [IDA]
- negative regulation of glial cell apoptotic process [IDA]
- negative regulation of natural killer cell chemotaxis [IDA]
- negative regulation of neuron apoptotic process [IDA]
- organ morphogenesis [TAS]
- positive regulation of T cell activation [ISS]
- positive regulation of apoptotic cell clearance [ISS]
- positive regulation of calcium ion import [IDA]
- positive regulation of nitric-oxide synthase biosynthetic process [ISS]
- protein kinase B signaling [IMP]
- protein phosphorylation [TAS]
- regulation of cell shape [IDA]
- response to bacterium [IEP]
- signal transduction [NAS]
- viral genome replication [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CXCL17
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
Chemokines orchestrate leukocyte trafficking and function in health and disease. Heterophilic interactions between chemokines in a given microenvironment may amplify, inhibit, or modulate their activity; however, a systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting and surface plasmon resonance to obtain a comprehensive map of chemokine-chemokine interactions and to confirm their specificity. Structure-function analyses ... [more]
Quantitative Score
- 0.158 [Relative OD]
Throughput
- High Throughput
Additional Notes
- averages of 2-4 experiments were used
- interaction assayed using densitometry analysis of bidirectional immunoligand blotting in which the bait protein was immobilized on a membrane, the prey protein was added in solution, prey was detected by specific antibody, and the densitometric optical density (OD) was normalized for area and reported as a ratio of a corresponding positive control
- interactions were considered positive if this relative ratio was equal to or greater than 0.05
Curated By
- BioGRID