Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase.

Wu Y, Dowbenko D, Spencer S, Laura R, Lee J, Gu Q, Lasky LA

PTEN/MMAC is a phosphatase that is mutated in multiple human tumors. PTEN/MMAC dephosphorylates 3-phosphorylated phosphatidylinositol phosphates that activate AKT/protein kinase B (PKB) kinase activity. AKT/PKB is implicated in the inhibition of apoptosis, and cell lines and tumors with mutated PTEN/MMAC show increased AKT/PKB kinase activity and resistance to apoptosis. PTEN/MMAC contains a PDZ domain-binding site, and we show here that ... [more]

J. Biol. Chem. Jul. 14, 2000; 275(28);21477-85 [Pubmed: 10748157]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BAI1 MAGI3	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Stephenson JR (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MAGI3

Gene Ontology Biological Process

Gene Ontology Molecular Function

guanylate kinase activity [NAS]protein binding [IPI]

Gene Ontology Cellular Component

BAI1